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one site–specific binding fitting model graphpad prism version 10.2.1  (GraphPad Software Inc)


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    Structured Review

    GraphPad Software Inc one site–specific binding fitting model graphpad prism version 10.2.1
    One Site–Specific Binding Fitting Model Graphpad Prism Version 10.2.1, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+model+fitting/one+site+specific+binding+fitting+model+graphpad+prism+version+10+2+1/pm40649798-258-20-24
    Average 90 stars, based on 1 article reviews
    one site–specific binding fitting model graphpad prism version 10.2.1 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Binding Assay:

    Article Title: RNA-triggered CRISPR-Cas12a2 Preferentially and Cooperatively Cleaves Collateral DNA
    Article Snippet: .. Binding isotherms were generated by fitting the data to a one-site total binding model in GraphPad Prism: .. Active ternary complex was first prepared by combining 2.0 µM Cas12a2, 1.0 µM crRNA and 100 nM target RNA in 1x NEB 3.1 buffer (50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM MgCl2, 100 ug/mL bovine serum albumin (BSA)) as a 2x master mix and incubated at room temperature for 30 minutes after the addition of both crRNA and target RNA as described previously ( ).

    Article Title: Single hormone or synthetic agonist induce Gs/Gi coupling selectivity of EP receptors via distinct binding modes and propagating paths
    Article Snippet: For EP4-Gαs/EP4-Gαi fusion protein, the KHi and KLo were calculated same as above according to a two-site binding model performed by GraphPad Prism 7 to indicate the characteristics of high affinity (KHi) and low affinity (KLo) of EP4-Gαs/EP4-Gαi fusion protein. .. In parallel, the corresponding fractions of the KHi and KLo were generated by the two-site binding model curve fitting using GraphPad Prism 7 (GraphPad Software Inc.). ..

    Generated:

    Article Title: RNA-triggered CRISPR-Cas12a2 Preferentially and Cooperatively Cleaves Collateral DNA
    Article Snippet: .. Binding isotherms were generated by fitting the data to a one-site total binding model in GraphPad Prism: .. Active ternary complex was first prepared by combining 2.0 µM Cas12a2, 1.0 µM crRNA and 100 nM target RNA in 1x NEB 3.1 buffer (50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM MgCl2, 100 ug/mL bovine serum albumin (BSA)) as a 2x master mix and incubated at room temperature for 30 minutes after the addition of both crRNA and target RNA as described previously ( ).

    Article Title: Single hormone or synthetic agonist induce Gs/Gi coupling selectivity of EP receptors via distinct binding modes and propagating paths
    Article Snippet: For EP4-Gαs/EP4-Gαi fusion protein, the KHi and KLo were calculated same as above according to a two-site binding model performed by GraphPad Prism 7 to indicate the characteristics of high affinity (KHi) and low affinity (KLo) of EP4-Gαs/EP4-Gαi fusion protein. .. In parallel, the corresponding fractions of the KHi and KLo were generated by the two-site binding model curve fitting using GraphPad Prism 7 (GraphPad Software Inc.). ..

    other:

    Article Title: Bioluminescence assay of lysine deacylase sirtuin activity.
    Article Snippet: KD values were estimated by fitting data to one site-specific binding model on GraphPad. (D) Kinetic parameters of furimazine with LgBiT and acylated peptides were estimated by fitting data to a hyperbolic equation. (E) Table of kinetic parameters shown in (C) and (D).

    Article Title: Tubulin polyglutamylation differentially regulates microtubule-interacting proteins.
    Article Snippet: Fitting these intensities with a onesite-specific binding model (GraphPad Prism 9) yielded dissociation constants of 0.48 0.1 μM (mean SD, N = 3 experiments with tubulin from independent purifications) for wild-type, that is, glutamylated microtubules, and 0.71 0.2 μM (mean SD, N = 3) for Ttll1−/−Ttll7−/−, that is, non-glutamylated microtubules (Figs 2E and EV2D).

    Article Title: Substrate promiscuity of inositol 1,4,5-trisphosphate kinase driven by structurally-modified ligands and active site plasticity
    Article Snippet: Fitting of the binding of 2-FITC-InsP 3 to IP3K-KD with a one-site binding model in GraphPad Prism v.6 (GraphPad Software Inc., San Diego, USA) yielded a K d of 68 nM.

    Article Title: Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae.
    Article Snippet: Data fitting using One-site-specific binding model was performed using GraphPad Prism.

    Article Title: Limited high-throughput screening compatibility of the phenuivirus cap-binding domain.
    Article Snippet: Fitting the data to a single site binding model using GraphPad Prism 9.5 resulted in a KD of 55 μM ± 7 μM, which is in a similar range as the previously determined KD of 138 μM ± 17 μM by ITC for SFTSV CBD and unlabeled m7GTP10.

    Article Title: Limited high-throughput screening compatibility of the phenuivirus cap-binding domain
    Article Snippet: Fitting the data to a single site binding model using GraphPad Prism 9.5 resulted in a K D of 55 μM ± 7 μM, which is in a similar range as the previously determined K D of 138 μM ± 17 μM by ITC for SFTSV CBD and unlabeled m GTP .



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